Q40 NADP+ & NADPH Blood Kit (RUO)
Q-NAD Blood NADP+ and NADPH Assay kit
Quantitative 40-sample assay kit for blood
Lead time approx. 2 months
| Catalog number / name | RUO_004 / Q-NAD Blood NADP+ and NADPH Assay kit |
| Detection method | Colorimetric (OD570-573nm) |
| Assay type | Quantitative |
| Samples type | Whole blood |
| Species | All |
| Sample volume | 120 μL (with K2 EDTA or heparin) |
| Metabolites measured | NADP+, NADPH |
| Detection Limit | 400 nM for NADP+ and 300 nM NADPH in the whole blood |
| Assay Length | < 1h |
| Size | 192 reactions |
| Number of samples | 40 (measured in duplicates) |
| Storage | Store reagents at -80°C |
| Intended use | RUO |
| Shelf Life | 12 months from the production date |
- Find the Instructions for Use under Q-NAD Blood NADP+ and NADPH Assay kit / Q40 NADP+ & NADPH Blood Kit (RUO) here.
- Certificate of Analysis is available upon request.
Please contact your NADMED representative or email us at info@nadmed.com. We will be happy to provide it.
- If you experience any issues, please refer to the troubleshooting guide.
Key Features
- Highly accurate and sensitive.
- Easy to handle. One step metabolite extraction is followed by separate measurement of NADP+ and NADPH in the extract. Detection time is less than 10 minutes.
- High throughput. Can be easily adopted into high-throughput 96-well plate assay to analyze hundreds of samples per day.
Principles
The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NADP+ and NADPH metabolites are extracted together from a blood sample in a single step. Then the extract is divided into two parts revealing both NADP+ and NADPH concentrations by removing one metabolite and stabilizing the other.
Reagents Provided
- EXTRACTION BUFFER A
- NADP+ STABILIZER
- NADPH STABILIZER
- NADP+ STANDARD STOCK
- NADPH STANDARD STOCK
- ASSAY BUFFER P
- ASSAY COLOR REAGENT
- NADP ENZYME
- STOP SOLUTION
- POSITIVE CONTROL
- DEIONIZED WATER
Storage
If you are storing the kits for more than one month, place them in a freezer set to -80°C. If you plan to run the assay within four weeks, the kits may be stored at stable -20°C.
Kit Requires
Multi-channel pipets. Two clear-bottom 96-well plates suitable for colorimetric assays, heat block with adjustable temperature (up to 80°C), spectrophotometric plate reader
Precautions and Warnings
For research use only.
The STOP SOLUTION may cause skin, eye and respiratory irritation. Avoid breathing in fumes.
ASSAY COLOR REAGENT may cause skin irritation. Handle with care, use gloves.
EXTRACTION BUFFER A can cause eye irritation. Handle with care, use goggles.
For more information, please refer to the Safety Data Sheet (SDS) for the blood kit and for more details on sample preparation, see the Blood preparation instructions, both available here.