Catalog number / name RUO_003 / Q-NAD Tissue/Cell NAD+ and NADH assay kit
Detection method Colorimetric (OD570-573nm)
Assay type Quantitative
Samples type Tissues / Cells
Species All
Guideline for sample volume 20 mg of tissue per 1 mL of extraction buffer 0.5 M cells per 100 μL of extraction buffer
Metabolites measured NAD+, NADH
Detection Limit 25 nM for NAD+ and 35 nM NADH in the cell/tissue extract
Assay Length < 1h
Size 192 reactions
Number of samples 40 (measured in duplicates)
Storage Store reagents at -80°C
Intended use RUO
Shelf Life 6 months

Instructions for use, Vs. 3, Last updated April 2024

Trouble shooting

Key Features


  • Highly accurate and sensitive.
  • Easy to handle. One step metabolite extraction is followed by separate measurement of NAD+ and NADH in the extract. Detection time is less than 10 minutes.
  • High throughput. Can be easily adopted into high-throughput 96-well plate assay to analyze hundreds of samples per day.


The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NAD+ and NADH metabolites are extracted together from a tissue or cell culture sample in a single step. Then the extract is divided into two parts revealing both NAD+ and NADH concentrations by removing one metabolite and stabilizing the other.

Reagents Provided   

Extraction BUFFER A, NAD+ stabilizing reagent, NADH stabilizing reagent, NAD+ standard stock, NADH standard stock, BUFFER C,
BUFFER D, Assay color reagent, Enzyme, Stop solution, Positive control

Kit Requires 

Multi-channel pipets. Two clear-bottom 96-well plates suitable for colorimetric assays, heat block with adjustable temperature (up to 80°C), spectrophotometric plate reader

Precautions and Warnings

For research use only.
The Stop Solution may cause skin, eye, and respiratory irritation. Avoid breathing in fumes.
Assay color reagent may cause skin irritation. Handle with care, use gloves.
BUFFER A can cause eye irritation. Handle with care, use googles.
Read more in the Safety Data Sheet (SDS) – vs.4, last modified January 2024

General Information

Proprietary name: Q-NAD tissue/cells: quantitative assay kit for NAD+ and NADH in tissues and cells
Catalog number: RUO_003, 40 samples (96-well format), 192 reactions

More details how to prepare samples in sample preparation document. Protocol for cell collection with NADMED lysis buffer you can find here (vs. 2, Last update April 2024).