Q40 NAD+ & NADH Tissue/Cells Kit (RUO)

Q-NAD Tissue/Cells NAD+ and NADH Assay Kit

Quantitative 40-sample assay kit for tissue and cells

Open Nadmed kit box

 

Catalog number / name

RUO_003 / Q-NAD Tissue/Cells NAD+ and NADH Assay kit

RUO_003_US / Q-NAD Tissue & Cells NAD+ and NADH Assay kit (US)
Detection method Colorimetric (OD570-573nm)
Assay type Quantitative
Samples type Tissue / Cells
Species All
Guideline for sample volume 20 mg of tissue per 1 mL of extraction buffer 0.5 M cells per 100 μL of extraction buffer
Metabolites measured NAD+, NADH
Detection Limit 25 nM for NAD+ and 35 nM NADH in the cell/tissue extract
Assay Length < 1h
Size 192 reactions
Number of samples 40 (measured in duplicates)
Storage Store reagents at -80°C
Intended use RUO
Shelf Life 12 months from the production date

  • Find the Instructions for Use under Q-NAD Tissue/Cells NAD+ and NADH Assay kit / Q40 NAD+ & NADH Tissue/Cells Kit (RUO) here.

  • Certificate of Analysis is available upon request.
    Please contact your NADMED representative or email us at info@nadmed.com. We will be happy to provide it.

Key Features

 

  • Highly accurate and sensitive.
  • Easy to handle. One step metabolite extraction is followed by separate measurement of NAD+ and NADH in the extract. Detection time is less than 10 minutes.
  • High throughput. Can be easily adopted into high-throughput 96-well plate assay to analyze hundreds of samples per day.

Principles

The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NAD+ and NADH metabolites are extracted together from a tissue or cell culture sample in a single step. Then the extract is divided into two parts revealing both NAD+ and NADH concentrations by removing one metabolite and stabilizing the other.

Reagents Provided   

  • EXTRACTION BUFFER B
  • NAD+ STABILIZER
  • NADH STABILIZER
  • POSITIVE CONTROL
  • SOLUBILIZATION BUFFER D
  • STOP SOLUTION
  • ASSAY BUFFER C
  • ASSAY COLOR REAGENT
  • NAD+ STANDARD STOCK
  • NADH STANDARD STOCK
  • NAD ENZYME

Storage

If you are storing the kits for more than one month, place them in a freezer set to -80°C. If you plan to run the assay within four weeks, the kits may be stored at stable -20°C.

Kit Requires 

Multi-channel pipets. Two clear-bottom 96-well plates suitable for colorimetric assays, heat block with adjustable temperature (up to 80°C), spectrophotometric plate reader

Precautions and Warnings

For research use only.
The STOP SOLUTION may cause skin, eye, and respiratory irritation. Avoid breathing in fumes.
ASSAY COLOR REAGENT may cause skin irritation. Handle with care, use gloves.
EXTRACTION BUFFER B can cause eye irritation. Handle with care, use goggles.

For more information, please refer to the Safety Data Sheet (SDS) for the tissue/cells kit.

For more details on sample preparation, see the Tissue and Cells preparation instructions. If you are using the NADMED lysis buffer, please follow the Protocol for cell collection with NADMED lysis buffer. 

The documents are available here.