Catalog number / name RUO_001 / Q-NAD Blood NAD+ and NADH assay kit
Detection method Colorimetric (OD570-573nm)
Assay type Quantitative
Samples type Whole blood
Species All
Sample volume 120 μL (with K2 EDTA or heparin)
Metabolites measured NAD+, NADH
Detection Limit  330 nM for NAD+ and 119 nM NADH in the whole blood
Assay Length < 1h
Size 192 reactions
Number of samples 40 (measured in duplicates)
Storage Store reagents at -80°C
Intended use For Research Use Only
Shelf Life 6 months


Instructions for use – Vs. 5, last modified April 2024

Trouble shooting

Certificate of Analysis




    Key Features

    • Highly accurate and sensitive. Quantitation limit is 330 nM for NAD+ and 119 nM NADH in whole blood measured in two 96-well plate assays.
    • Easy to handle. One step metabolite extraction is followed by separate measurement of NAD+ and NADH in the extract. Detection time is less than 10 minutes.
    • High throughput. Can be easily adopted into high-throughput 96-well plate assay to analyze hundreds of samples per day.


    The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NAD+ and NADH metabolites are extracted together from a blood sample in a single step. Then the extract is divided into two parts revealing both NAD+ and NADH concentrations by removing one metabolite and stabilizing the other.

    Reagents Provided   

    Extraction BUFFER A, NAD+ stabilizing reagent, NADH stabilizing reagent, NAD+ standard stock, NADH standard stock, Assay BUFFER C, Assay color reagent, Enzyme, Stop solution, Positive control

    Kit Requires 

    Multi-channel pipets. Two clear-bottom 96-well plates suitable for colorimetric assays, heat block with adjustable temperature (up to 80°C), spectrophotometric plate reader

    Precautions and Warnings

    For research use only by trained personnel.
    The Stop Solution may cause skin, eye, and respiratory irritation. Avoid breathing in fumes.
    Assay color reagent may cause skin irritation. Handle with care, use gloves.
    BUFFER A can cause eye irritation. Handle with care, use googles.

    Read more in Q-NADMED Safety Data Sheet (SDS)

    More details how to prepare samples in the sample preparation document – vs.2, last modified September 2023.