Q16 NAD+ & NADH Small Blood Kit (RUO)

Q-NAD Blood NAD+ and NADH Assay kit – SMALL

 

Quantitative 16-sample assay kit for blood

Open Nadmed kit box

 

 

Catalog number / name

RUO_001_S / Q-NAD Blood NAD+ and NADH Assay kit – SMALL

RUO_001_US_S / Q-NAD Blood NAD+ and NADH Assay kit (US) – SMALL
Detection method Colorimetric (OD570-573nm)
Assay type Quantitative
Samples type Whole blood
Species All
Sample volume 120 μL (with K2 EDTA or heparin)
Metabolites measured NAD+, NADH
Detection Limit  330 nM for NAD+ and 119 nM NADH in the whole blood
Assay Length < 1h
Size 96 reactions
Number of samples 16 (measured in duplicates)
Storage Store reagents at -80°C
Intended use For Research Use Only
Shelf Life 12 months from the production date
  • Find the Instructions for Use under Q-NAD Blood NAD+ and NADH Assay kit – SMALL / Q16 NAD+ & NADH Small Blood Kit (RUO) here.

 

  • Certificate of Analysis is available upon request.
    Please contact your NADMED representative or email us at info@nadmed.com. We will be happy to provide it.

 

 

INSTRUCTIONAL VIDEO

    Key Features

    • Highly accurate and sensitive. Quantitation limit is 330 nM for NAD+ and 119 nM NADH in whole blood measured in two 96-well plate assays.
    • Easy to handle. One step metabolite extraction is followed by separate measurement of NAD+ and NADH in the extract. Detection time is less than 10 minutes.
    • High throughput. Can be easily adopted into high-throughput 96-well plate assay to analyze hundreds of samples per day.

    Principles

    The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NAD+ and NADH metabolites are extracted together from a blood sample in a single step. Then the extract is divided into two parts revealing both NAD+ and NADH concentrations by removing one metabolite and stabilizing the other.

    Reagents Provided
     

    • EXTRACTION BUFFER A – SMALL
    • NAD+ STABILIZER
    • NADH STABILIZER
    • NAD+ STANDARD STOCK
    • NADH STANDARD STOCK
    • ASSAY BUFFER C – SMALL
    • ASSAY COLOR REAGENT – SMALL
    • NAD ENZYME
    • STOP SOLUTION
    • POSITIVE CONTROL
    • DEIONIZED WATER

    Storage

    If you are storing the kits for more than one month, place them in a freezer set to -80°C. If you plan to run the assay within four weeks, the kits may be stored at stable -20°C.

    Kit Requires 

    Multi-channel pipets. Two clear-bottom 96-well plates suitable for colorimetric assays, heat block with adjustable temperature (up to 80°C), spectrophotometric plate reader

    Precautions and Warnings

    For research use only by trained personnel.
    The STOP SOLUTION may cause skin, eye, and respiratory irritation. Avoid breathing in fumes.
    ASSAY COLOR REAGENT – SMALL may cause skin irritation. Handle with care, use gloves.
    EXTRACTION BUFFER A – SMALL can cause eye irritation. Handle with care, use goggles.

    For more information, please refer to the Safety Data Sheet (SDS) for the blood kit and for more details on sample preparation, see the Blood preparation instructions, both available here.