Q-NADMED KIT Blood

Quantitative assay kit for NAD+ and NADH in blood

 

Detection method Colorimetric (OD570-573nm)
Assay type Quantitative
Samples type Whole blood
Species All
Sample volume 120 μL (with EDTA or heparin)
Metabolites measured NAD+, NADH
Detection Limit  400 nM for NAD+ and 300 nM NADH in the whole blood
Assay Length < 1h
Size 192 reactions
Number of samples 40 (measured in duplicates)
Storage Store reagents at -80°C
Intended use CE-IVD
Shelf Life 6 months

Key Features

 

  • Highly accurate and sensitive. Quantitation limit is 400 nM for NAD+ and 300 nM NADH in whole blood measured in two 96-well plate assays.
  • Easy to handle. One step metabolite extraction is followed by separate measurement of NAD+ and NADH in the extract. Detection time is less than 10 minutes.
  • High throughput. Can be easily adopted into high-throughput 96-well plate assay to analyze hundreds of samples per day.

Intended Use

Q-NADMED Blood, an in-vitro diagnostic medical device, is an analytical assay kit for measurement of concentrations of NAD+ and NADH metabolites in human whole blood. The assay is quantitative. Intended users of the Q-NADMED assay kit are trained laboratory personnel.

Principals

The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NAD+ and NADH metabolites are extracted together from a blood sample in a single step. Then the extract is divided into two parts revealing both NAD+ and NADH concentrations by removing one metabolite and stabilizing the other.

Reagents Provided   

Extraction BUFFER A, NAD+ stabilizing reagent, NADH stabilizing reagent, NAD+ standard stock, NADH standard stock, Assay BUFFER C, Assay color reagent, Enzyme, Stop solution, Positive control

Kit Requires 

Multi-channel pipets. Two clear-bottom 96-well plates suitable for colorimetric assays, heat block with adjustable temperature (up to 80°C), spectrophotometric plate reader

Precautions and Warnings

For in vitro diagnostic use only by trained personnel.
The Stop Solution may cause skin, eye, and respiratory irritation. Avoid breathing in fumes.
Assay color reagent may cause skin irritation. Handle with care, use gloves.
BUFFER A can cause eye irritation. Handle with care, use googles.
Read more in Q-NADMED Safety Data Sheet (SDS)

General Information

Proprietary name: Q-NADMED Blood: quantitative assay kit for NAD+ and NADH in blood
Catalog number: IVC_001, 40 samples (96-well format), 192 reactions
IFU issued: May 2022

More details how to prepare samples in sample preparation document.