|Detection method||Colorimetric (OD570-573nm)|
|Samples type||Whole blood|
|Sample volume||120 μL (with EDTA or heparin)|
|Metabolites measured||NAD+, NADH|
|Detection Limit||400 nM for NAD+ and 300 nM NADH in the whole blood|
|Assay Length||< 1h|
|Number of samples||40 (measured in duplicates)|
|Storage||Store reagents at -80°C|
|Shelf Life||6 months|
- Highly accurate and sensitive. Quantitation limit is 400 nM for NAD+ and 300 nM NADH in whole blood measured in two 96-well plate assays.
- Easy to handle. One step metabolite extraction is followed by separate measurement of NAD+ and NADH in the extract. Detection time is less than 10 minutes.
- High throughput. Can be easily adopted into high-throughput 96-well plate assay to analyze hundreds of samples per day.
Q-NADMED Blood, an in-vitro diagnostic medical device, is an analytical assay kit for measurement of concentrations of NAD+ and NADH metabolites in human whole blood. The assay is quantitative. Intended users of the Q-NADMED assay kit are trained laboratory personnel.
The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NAD+ and NADH metabolites are extracted together from a blood sample in a single step. Then the extract is divided into two parts revealing both NAD+ and NADH concentrations by removing one metabolite and stabilizing the other.
Extraction BUFFER A, NAD+ stabilizing reagent, NADH stabilizing reagent, NAD+ standard stock, NADH standard stock, Assay BUFFER C, Assay color reagent, Enzyme, Stop solution, Positive control
Multi-channel pipets. Two clear-bottom 96-well plates suitable for colorimetric assays, heat block with adjustable temperature (up to 80°C), spectrophotometric plate reader
Precautions and Warnings
For in vitro diagnostic use only by trained personnel.
The Stop Solution may cause skin, eye, and respiratory irritation. Avoid breathing in fumes.
Assay color reagent may cause skin irritation. Handle with care, use gloves.
BUFFER A can cause eye irritation. Handle with care, use googles.
Read more in Q-NADMED Safety Data Sheet (SDS)
More details how to prepare samples in sample preparation document.