|Detection method||Colorimetric (OD570-573nm)|
|Samples type||Whole blood|
|Sample volume||120 μL (with K2 EDTA or heparin)|
|Metabolites measured||NADP+, NADPH|
|Detection Limit||400 nM for NADP+ and 300 nM NADPH in the whole blood|
|Assay Length||< 1h|
|Number of samples||40 (measured in duplicates)|
|Storage||Store reagents at -80°C|
|Shelf Life||6 months|
- Highly accurate and sensitive.
- Easy to handle. One step metabolite extraction is followed by separate measurement of NADP+ and NADPH in the extract. Detection time is less than 10 minutes.
- High throughput. Can be easily adopted into high-throughput 96-well plate assay to analyze hundreds of samples per day.
The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NADP+ and NADPH metabolites are extracted together from a blood sample in a single step. Then the extract is divided into two parts revealing both NAPD+ and NADPH concentrations by removing one metabolite and stabilizing the other.
Extraction BUFFER A, NAPD+ stabilizing reagent, NADPH stabilizing reagent, NADP+ standard stock, NADPH standard stock, Assay BUFFER C, Assay color reagent, Enzyme, Stop solution, Positive control
Multi-channel pipets. Two clear-bottom 96-well plates suitable for colorimetric assays, heat block with adjustable temperature (up to 80°C), spectrophotometric plate reader
Precautions and Warnings
For research use only.
The Stop Solution may cause skin, eye and respiratory irritation. Avoid breathing in fumes.
Assay color reagent may cause skin irritation. Handle with care, use gloves.
BUFFER A can cause eye irritation. Handle with care, use googles.
Read more in Q-NAD Safety Data Sheet (SDS)
Proprietary name: Q-NADP Blood: quantitative assay kit for NADP+ and NADPH in blood
More details how to prepare samples in sample preparation document.